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jara_j wrote:There are alvays doubt about encapsulation of home made LVC. If you make LVC with Ascorbic Acid, you can easy test encapsulation by this method. You only need precision scales, sodium bicarbonate and pH meter (or pH stick).
1. Pour out sample of LVC about 10g, record the exact weight (W1).
2 Add deistilled water about 50g, record exact weight of added water (W2).
3. Gently mix and then let Liposoms go down. Pour out water with dissolved non liposomal AA into another glass. You can easily separate up to 90% of it from liposomes. Record weight of separated solution (W3).
4. Measure pH of separated solution and in small steps add sodium bicarbonate and well mix. Wait until it dissolves. Do until pH goes over 7. Record weight of added sodium bicarbonate (W4).
5. Count amount of non encapsulated AA as W=2,1*W4*W2/W3. Count encapsulation as 1-W/(W1*original percentage of AA/100).
Mine homemade LVC gets result about 50% or more. Batch I made Yesterday get 65%. Theoretical geometrical maximum is 74%.
My Zesterday data:
W1=8.1g, W2=39.4g, W3=35.8g, W4=0.222g, percentage of AA in LVC is 17.9%.
Simnple method so why don't use it to check our products?
Note: You have to separate liposomes out, because they would be destroyed by sodium bicarbonate crystals and all will fail.
Jaromir
jeff wrote:Thank you for your reply. I appreciate that the other components of lecithin may contribute to encapsulation. Putting that aside for the moment, can you think of any reason not to increase the amount of lecithin to approximate the 9.4% PC in the Livon formula (f the mixture becomes too thick, just add water, right?)
BrightSideOfLife wrote:I have used your figures and cannot get the encapsulation that you say you accomplished for the provided data.
Encapsulation comes out as 0.33582974899829346735914362809071 and none encapsulated AA (W) gives me 0.51308044692737430167597765363128 this is assuming that the 2,1 is 2.1
Is there an error somewhere? I have calculated it many times but get no where close to 65 (%).
This would need to work correctly before anyone is going to use it.
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